Low-Grade Systemic Inflammation Interferes with Anabolic and Catabolic Characteristics of the Aged Human Skeletal Muscle

Figure 2

Evidence for impaired anabolic potential in older adults with chronic LGSI. (a) Quantification of phosphorylated ribosomal protein S6 (rpS6) ( in ESI and 10 in control), c-Myc ( in ESI and 6 in control), phosphorylated transcription initiation factor-1A (TIF-1A) ( in ESI and 7 in control), L-type amino acid transporter 1 (LAT1) ( in ESI and 9 in control), and sodium-coupled neutral amino acid transporter 2 (SNAT2/SLC38A2) ( in ESI and 9 in control) by densitometry (all results are expressed as ratio of each protein to GAPDH). (b) Representative western blots for rpS6, c-Myc, TIF-1A, LAT1, and SNAT2/SLC38A2. (c) Quantification of the Pax7⁺ cells per myofiber in skeletal muscle ( in ESI and 8 in control). (d) Representative images of staining against Pax7 antibody (i: Pax7/Laminin/DAPI stain of a muscle cross-section; ii–vi: channel views of merge Pax7/Laminin (ii), Pax7/DAPI and single Pax7 (iv), Laminin (v), and DAPI (vi). ^#Significant difference between groups (). All data are presented as .

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