Polymethoxyflavones in Citrus Regulate Lipopolysaccharide-Induced Oscillating Decay of Circadian Rhythm Genes by Inhibiting Nlrp3 Expression

Figure 1

Regulation of citrus flavonoids on circadian rhythms of BV-2 cells: (a) HPLC chromatogram of flavonoid extracts from 7 varieties of citrus; (b) flavonoid contents in flavedo of 7 citrus varieties; (c) cell viabilities of BV-2 cells under different citrus flavonoid extracts and LPS treatments measured using a cell counting kit-8 assay; (d) effects of citrus flavonoid extracts on the expression of circadian rhythm gene Per2 using luciferase bioluminescence reporters; (e) effects of citrus flavonoid extracts on LPS-disturbed expression of circadian gene Per2. FJ: Fuju; ZJOG: Zijinougan; YL: Youliang; DETC: Daetiancheng; MWG: Manwengan; JWPTY: Jiweiputaoyou; MBWD: Mabuwendan. Data is presented as the (). indicates significant difference between flavonoid treatments compared to the DMSO solvent control ().

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