Polymethoxyflavones in Citrus Regulate Lipopolysaccharide-Induced Oscillating Decay of Circadian Rhythm Genes by Inhibiting Nlrp3 Expression

Figure 4

Regulation of Nlrp3 expression by citrus flavonoids in BV-2 cells: (a) regulation of Nlrp3 inflammasome-related genes, Nlrp3, IL-1β, ASC, and Caspase 1, by citrus flavonoids (indicated in the color-coded legend to the right of panel), as measured by qRT-PCR; (b) ELISA detection of Nlrp3 inflammasome-related proteins regulated by flavonoids (indicated by the color-coded legend to the right of panel). The pretreatment concentration was 10 μM for nobiletin, sinensetin, isosinensetin, tangeretin, 5-demethylnobiletin, and gardenin B; 160 μM for diosmetin, hesperetin, apigenin, and naringenin; and 320 μM for isovitexin, vitexin, vicenin-2, hesperidin, diosmin, didymin, narirutin, isorhoifolin, eriocitrin, neohesperidin, neodiosmin, poncirin, naringin, rhoifolin, and neoeriocitrin. Data is presented as the (). indicates significant difference between flavonoid treatments compared to the LPS treatment ().

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