MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer’s Disease

Figure 1

A behavioral analysis of wild-type, App^{NL-G-F/NL-G-F}, and App^{NL-G-F/NL-G-F}·Mutyh^-/- mice. (a) Timelines for the behavioral analyses. After being individually housed for at least seven days, mice were randomly assigned to group A or group B for the behavioral analyses. HC = spontaneous locomotor activity in the home cage; OF = open-field test; NOR = novel object recognition test. (b–e) Spontaneous locomotor activity. Locomotor counts in the dark phase averaged over three days (8:00 pm to 8:00 am) are shown. Wild-type (Wt), App^{NL-G-F/NL-G-F} (NL-G-F), and App^{NL-G-F/NL-G-F}·Mutyh^-/- (NL-G-F·Mutyh). (f–i) Open-field test. The total travel distance (cm) during 5 min is shown. (j–m) Novel object recognition test. The preference index is shown. (b, f, j) Six-month-old male mice. (c, g, k) Six-month-old female mice. (d, h, l) Twelve-month-old male mice. (e, i, m) Twelve-month-old female mice. The data are expressed as the . -15 per group. Statistical analyses were performed with a one-way ANOVA (the value is shown in each bar graph) followed by post hoc Student’s -test. . In six-month-old female mice, a one-way ANOVA revealed marginal significance in spontaneous locomotor activity (c) and the novel object recognition test (k), so Hsu’s MCB was performed. ^#.

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