MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer’s Disease

Figure 4

Immunohistochemical detection of 8-oxoG in human brains. (a) Validation of 8-oxoG immunoreactivity detected by the anti-8-oxo-dG antibody. Sections of the non-AD hippocampus pretreated only with RNase were subjected to immunohistochemistry with the anti-8-oxo-dG antibody and the antibody preadsorbed by 8-oxo-dG (anti-8-oxo-dG + 8-oxo-dG) or 2-deoxyguanosine (anti-8-oxo-dG + dG). Cytoplasmic immunoactivities were observed in the neurons of the non-AD hippocampus (left). Preadsorption of the antibody with 8-oxo-dG (middle) but not with dG (right) abolished the immunoactivity. for the full image and 20 μm for magnified images. (b) Immunohistochemical detection and quantification of 8-oxoG in mitochondrial DNA of the hippocampus. The sections were pretreated only with RNase. (c) Immunohistochemical detection and quantification of 8-oxoG in nuclear DNA of the CA1 field (CA1), CA4 field (CA4), and DG. The sections were pretreated with RNase followed by HCl. Nuclear 8-oxoG immunoreactivity-positive neuron (i). . The data are shown as boxplots with dots and whiskers indicating the minimum and maximum (). Statistical analyses were performed with Wilcoxon’s rank sum test, and the value is shown in each graph.

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