MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer’s Disease

Figure 6

The Aβ deposition in the App^{NL-G-F/NL-G-F} brain was not changed by MUTYH deficiency. (a–d) Immunohistochemistry with anti-Aβ (82E1) in the six-month-old male (a) and female (c) brain. App^{NL-G-F/NL-G-F} (NL-G-F) and App^{NL-G-F/NL-G-F}·Mutyh^-/- (NL-G-F·Mutyh). . (b, d) The Aβ-positive area in the hippocampus, including the CA1 field and DG (CA1 + DG) and cortex (Ctx), was measured in every 5 coronal sections (4 sections per mouse; bregma -1.555 to -2.155 mm). Statistical analyses were performed with Student’s -test, and the value is shown in each bar graph (, ). (e, f) The results of a dot blot analysis of SDS-insoluble/formic acid (FA)-extractable Aβ in the six-month-old female mouse hippocampus (e) and cortex (f) with anti-Aβ (6E10) are shown. Top panels: A1 to A12, six-month-old female App^{NL-G-F/NL-G-F} samples; B1 to B12, six-month-old female App^{NL-G-F/NL-G-F} ·Mutyh^-/- samples; and C1 to C3, twelve-month-old female App^{NL-G-F/NL-G-F} samples as a control. Bottom panels: the intensity of SDS-insoluble/FA-extractable Aβ was normalized to the brain weight, and the values are relative to the average intensity of six-month-old female App^{NL-G-F/NL-G-F} samples. Statistical analyses were performed with Student’s -test, and the value is shown in each bar graph (, ).

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