MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer’s Disease

Figure 9

MUTYH deficiency decreased the accumulation of 8-oxoG in six-month-old female App^{NL-G-F/NL-G-F} mouse brains. (a) Immunohistochemical detection of 8-oxoG in mitochondrial DNA with RNase pretreatment. (b) Immunohistochemical detection of 8-oxoG in nuclear DNA with RNase and HCl pretreatment. for the hippocampus and 200 μm for the cortex. Wild-type (Wt), App^{NL-G-F/NL-G-F} (NL-G-F), and App^{NL-G-F/NL-G-F}·Mutyh^-/- (NL-G-F·Mutyh). (c, d) Quantitative measurement of mitochondrial (c) and nuclear (d) 8-oxoG in the brain. (e) Diagram depicting the four studied zones in the hippocampus: CA1-sp, entire CA1 field (CA1), ML, and GCL of DG. These fields were measured in every 5 coronal sections (4 sections per mouse; bregma -1.555 to -2.155 mm), and the results are shown as the (). Statistical analyses were performed with a one-way ANOVA ( value shown in each bar graph), followed by the post hoc Tukey-Kramer HSD test. .

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