Inhibition of Growth of Colon Tumors and Proliferation of HT-29 Cells by Warburgia ugandensis Extract through Mediating G₀/G₁ Cell Cycle Arrest, Cell Apoptosis, and Intracellular ROS Generation

Figure 2

Effect of WUD on ROS production of CRC cells. HT-29 cells (a, b) and HCT116 cells (d, e) were treated with WUD for 24 h; then, the cells were stained with DCFH-DA. The fluorescent intensity of DCF was detected by a microplate reader (a, d) and a cell imaging multi-mode reader (b, e). The fluorescence values of DCF were normalized with the SRB values of the same samples. HT-29 cells (c) and HCT116 cells (f) were pre-treated with NAC for 1 h and then treated with WUD for 24 h. Cell growth inhibition ratio was determined by SRB assay. Data were . . ns: not significant.

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