Research Article

Inhibition of Growth of Colon Tumors and Proliferation of HT-29 Cells by Warburgia ugandensis Extract through Mediating G0/G1 Cell Cycle Arrest, Cell Apoptosis, and Intracellular ROS Generation

Figure 4

Effect of WUD on PI3K, Akt, and GSK3β expression. HT-29 cells were treated with 0, 5, and 10 μg/mL WUD for 24 h; then, the cells were subjected to western blot analysis and detected by PI3K, Akt, p-Akt (Ser473), GSK3β, and p-GSK3β (Ser9) antibodies. GAPDH was used as a loading control.