hsa-miR-199b-3p Prevents the Epithelial-Mesenchymal Transition and Dysfunction of the Renal Tubule by Regulating E-cadherin through Targeting KDM6A in Diabetic Nephropathy

Figure 1

The promotive role of KDM6A in regulating EMT in HK2 cells. (a) Representative images of migration and the graphs of wound area quantification in HK2 cells cultured in HG or Mann at 0 h and 48 h. . (b) Western blot analysis of TGFβ, E-cadherin, vimentin, N-cadherin, and KDM6A in HK2 cells cultured in HG or Mann for 72 h. . (c) The relative level of TGFβ, E-cadherin, vimentin, N-cadherin, and KDM6A expression by qPCR in HK2 cells cultured in HG or Mann for 72 h. . (d) Representative images of migration and the graphs of wound area quantification in HK2 cells cultured in HG treatment with GSK-J4 (40μM) or equivoluminal ddH₂O (NC) at 0 h and 48 h. . (e) Western blot analysis of TGFβ, vimentin, N-cadherin, and E-cadherin in HK2 cells cultured in HG treatment with GSK-J4 or ddH₂O for 72 h. . (f) The relative level of TGFβ, vimentin, N-cadherin, and E-cadherin expression by qPCR in HK2 cells cultured in HG treatment with GSK-J4 or ddH₂O for 72 h. . values is presented. , , and (Student’s -test).