hsa-miR-199b-3p Prevents the Epithelial-Mesenchymal Transition and Dysfunction of the Renal Tubule by Regulating E-cadherin through Targeting KDM6A in Diabetic Nephropathy

Figure 2

The pivotal role of KDM6A in the progression of kidney injury in DN. (a) The relative level of KDM6A expression by qPCR in kidney tissue from C57BL/6 mice (WT-NC) and STZ-induced mice (WT-STZ) (at 8 weeks after the onset of diabetes). . (b) Representative images of KDM6A immunohistochemical staining in kidneys from WT-NC and WT-STZ. Scale bar, 200 μm. (c) The graphs showing quantification () of KDM6A immunohistochemical staining results in (b). . (d) Western blot analysis of KDM6A in the kidney from mice. . (e, g) Representative images of PAS staining and HE staining of kidneys from C57BL/6 mice with different treatments. STZ-induced C57BL/6 mice treated without (WT-STZ-NC) or with GSK-J4 (WT-STZ-G). Scale bar, 40 μm. Proximal tubule marked with red arrows and distal tubule marked with black arrows. (f, h) Representative images of area amplification marked with red boxes in (e) and (g), respectively. Scale bar, 20 μm. Proximal tubule marked with red arrows and distal tubule marked with black arrows. Lumen of proximal tubules marked with black asterisk. (i–l) The graphs showing the results of ACR, BUN, Cr, and proteinuria in different treated mice at 8 weeks after the onset of diabetes. . (m, n) Representative images of TGFβ and E-cadherin immunohistochemical staining in kidneys of different treated mice. Scale bar, 50 μm. (o, p) The graphs showing quantification () of TGFβ and E-cadherin immunohistochemical staining results in (l) and (m), respectively. . (q) Western blot analysis of TGFβ, E-cadherin, vimentin, and N-cadherin in the kidney from mice. . values is presented. , , and (Student’s -test in (a), (c), and (d); one-way ANOVA in (i)–(l) and (o)–(q).