IRS-2/Akt/GSK-3<i>β</i>/Nrf2 Pathway Contributes to the Protective Effects of Chikusetsu Saponin IVa against Lipotoxicity

<div>The antioxidative effects of CHS <i>in vitro</i>. (a) The effects of CHS on palmitate-induced ROS production in <i>β</i>TC3 cells. Cells were pretreated with 10-40 <i>μ</i>M CHS or Gli for 24 h, followed by incubation with PA for 24 h, then intracellular ROS levels assayed with DHE and DCF-DA probe as described in the experimental section <a href="https://static.hindawi.com/articles/omcl/volume-2021/8832318/figures/#sec4.5">4.5</a>. Representative pictures from three independent experiments are shown (×400). MDA (b), SOD (c), GPx (d), and GSH (e) were measured after CHS and PA treatments by relative kits. 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: IRS-2/Akt/GSK-3<i>β</i>/Nrf2 Pathway Contributes to the Protective Effects of Chikusetsu Saponin IVa against Lipotoxicity 

Oxidative Medicine and Cellular Longevity

IRS-2/Akt/GSK-3β/Nrf2 Pathway Contributes to the Protective Effects of Chikusetsu Saponin IVa against Lipotoxicity

Figure 5