Differential ROS-Mediated Phosphorylation of Drp1 in Mitochondrial Fragmentation Induced by Distinct Cell Death Conditions in Cerebellar Granule Neurons

Figure 5

Effect of mtROS in the activation of Drp1 induced by K5 and ST. The levels of total Drp1 and Drp1 phosphorylated at Ser616 (p-Drp1) were evaluated in a temporal course in lysates of CGN pretreated for 30 min with the mitochondrial antioxidant MitoTEMPO under death conditions. The levels of the protein were determined by Western blot analysis as indicated in Materials and Methods. (a) Levels of p-Drp1 (Ser616) from CGN treated with K5. (b) Levels of p-Drp1 from CGN pretreated with MitoTEMPO and treated with K5. (c) Levels of p-Drp1 (Ser616) from CGN treated with ST. (d) Levels of p-Drp1 from CGN pretreated with MitoTEMPO and treated with ST. GAPDH was used as loading control. The bars show the densitometric ratio between p-Drp1 and Drp1 that were normalized to the control K25. Values are the of three individual experiments. vs. K25.

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