Apelin/APJ-Manipulated CaMKK/AMPK/GSK3<i>β</i> Signaling Works as an Endogenous Counterinjury Mechanism in Promoting the Vitality of Random-Pattern Skin Flaps

<div>The apelin/APJ axis was shut down after random flap operation. (a, c) Western blot results of skin tissue suggest that apelin, APJ, CD34, VEGF, SOD2, and HO-1 were significantly downregulated and apoptosis was upregulated (Bcl2 decreased while Bax increased) after random flap operation. (b, d) IHC staining indicated that the apelin- and APJ-positive area almost halved (scale bar: 50 <i>μ</i>m). (e) Immunofluorescence colocalization showed that the codistribution of apelin and APJ in skin microvessels decreased significantly after operation (scale bar: 25 <i>μ</i>m). 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Oxidative Medicine and Cellular Longevity

fig1

Figure 1

Figure 1: Apelin/APJ-Manipulated CaMKK/AMPK/GSK3<i>β</i> Signaling Works as an Endogenous Counterinjury Mechanism in Promoting the Vitality of Random-Pattern Skin Flaps 

Figure 1 | Apelin/APJ-Manipulated CaMKK/AMPK/GSK3β Signaling Works as an Endogenous Counterinjury Mechanism in Promoting the Vitality of Random-Pattern Skin Flaps 