The Activation of ROS/NF-κB/MMP-9 Pathway Promotes Calcium-Induced Kidney Crystal Deposition

Figure 4

Effects of NAC and BAY 11-7082 on MMP-9 and osteoblastic-related protein expressions in NRK-52E cells exposed to calcium. (a) Intracellular p-p65 distribution was detected by immunofluorescence assay after pretreatment with or without NAC (magnification 1000x). (b) The expressions of NF-κB signaling pathway proteins were detected by western blot assay in NRK-52E cells with or without NAC pretreatment. Quantitative analysis of the densities of western blot bands. (c) The expressions of MMP-9, OPN, RUNX2, and E-cadherin proteins were detected by western blot assay in NRK-52E cells with or without NAC pretreatment. Quantitative analysis of the densities of western blot bands. (d) The expressions of MMP-9, OPN, RUNX2, and E-cadherin mRNAs were detected by q-PCR assay in NRK-52E cells with or without NAC pretreatment. (e) Intracellular p-p65 distribution was detected by immunofluorescence assay after pretreatment with or without BAY 11-7082 (magnification 1000x). (f) The expressions of MMP-9, OPN, RUNX2, and E-cadherin mRNAs were detected by q-PCR assay in NRK-52E cells with or without BAY 11-7082 pretreatment. (g) The expressions of MMP-9, OPN, RUNX2, and E-cadherin proteins were detected by western blot assay in NRK-52E cells with or without BAY 11-7082 pretreatment. Quantitative analysis of the densities of western blot bands. versus the control group, versus the control group.

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