Research Article

Stimulation of Sigma-1 Receptor Protects against Cardiac Fibrosis by Alleviating IRE1 Pathway and Autophagy Impairment

Table 1

The reaction system of reverse transcript.

ReagentVolume (μL)

buffer4.0
Primer mixture1.0
ReverTra Ace1.0
1.5 μg RNAX
RNAase-free ddH2O(14-X)
Total volume:20 μL