Anti-inflammatory Effects of S. cumini Seed Extract on Gelatinase-B (MMP-9) Regulation against Hyperglycemic Cardiomyocyte Stress

Figure 3

Expression analysis of gelatinase-B. (a) RT-PCR studies: Lane 1: control; Lane 2: glucose induced (GI); Lane 3: glucose induced+S. cumini methanol seed extract (GI+MSE) treated; Lane 4: MSE-treated alone (MSE); Lane 5: glucose induced+gallic acid treated (GI+GAL); Lane 6: gallic acid alone (GAL) (). Expression levels of MMP-9 were obtained by qRT-PCR and normalized against β-actin. Fold changes were shown as histogram (). (b) Immunocytochemistry for gelatinase-B: photomicrographs showing expression of MMP-9 in different experimental groups at 20x magnification. Scale bar 10 μm.