Anti-inflammatory Effects of S. cumini Seed Extract on Gelatinase-B (MMP-9) Regulation against Hyperglycemic Cardiomyocyte Stress

Figure 5

RT-PCR profiles of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in different experimental groups. Lane 1: control; Lane 2: glucose induced (GI); Lane 3: glucose induced+S. cumini methanol seed extract (GI+MSE) treated; Lane 4: MSE-treated alone (MSE); Lane 5: glucose induced+gallic acid treated (GI+GAL); Lane 6: gallic acid alone (GAL). (a) Semiquantitative PCR showed significant increase in expression for both the markers in the cells treated with high glucose (HG). S. cumini methanol seed extract (MSE) and gallic acid (GAL) treatment reduced it remarkably up to the control. (b) Results obtained by qRT-PCR (real-time PCR) were normalized against β-actin and represented by histogram ().

(a)

(b)