Sea Cucumber-Derived Peptides Alleviate Oxidative Stress in Neuroblastoma Cells and Improve Survival in C. elegans Exposed to Neurotoxic Paraquat

Figure 4

Sea cucumber-derived peptides reduce mitophagy induced by oxidative stress. (a) SH-SY5Y cells were grown in glass-bottom MatTek dishes until they reached partial confluency (50-70%). Next, cells were treated with 50 μM peptides or 50 μM GSH. Peptides were added together with SiR-Lysosome, a lysosomal marker. 24 hours after incubation, cellular oxidative stress was induced by treating cells with 1 mM H₂O₂ for 2 h at 37°C. Cells were then washed three times with PBS before the addition of rhodamine B (a mitochondrial stain) for 30 min at 37°C. After a further wash, SiR-Lysosome and rhodamine B fluorescence were detected by confocal microscopy. White arrows point to the mitochondria colocalised with lysosomes. (b) Quantification of the colocalisation of mitochondria and lysosomes was done by measuring Manders’ coefficient. Samples were compared with “no peptide,” and statistical significance was determined by Tukey one-way ANOVA. , , , and ; NS: not significant. Error bars show SD (). Experiments were repeated three times.

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