Melittin Ameliorates Endotoxin-Induced Acute Kidney Injury by Inhibiting Inflammation, Oxidative Stress, and Cell Death in Mice

Figure 3

Effects of melittin on plasma and renal levels of cytokines and NF-κB signaling cascade after LPS treatment. (a) Plasma TNF-α levels. (b) Plasma IL-6 levels. (c) The mRNA levels of TNF-α and IL-6 in kidney tissues. (d) Western blotting of TNF-α and IL-6 in kidney tissues. (e) Western blotting of IκB-α, p-IκB-α, NF-κB p65, and p-NF-κB p65 in kidney tissues. (f) Electrophoretic mobility shift assay for NF-κB DNA-binding activity in kidney tissues. compared with the vehicle-injected mice (Veh). ^## or ^### compared with the LPS-injected group (LPS).

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