Polygonatum sibiricum Polysaccharides Protect against MPP-Induced Neurotoxicity via the Akt/mTOR and Nrf2 Pathways

Figure 3

PSP inhibits MPP+-induced neuronal apoptosis by restoring the Akt/mTOR pathway. (a) Effect of PSP on the proliferation of N2a cells. The cells were treated with indicated concentrations of PSP for 1 d or 3 d, and cell viability was determined by CCK-8 assay (). (b) Western-blot analyses showed that PSP upregulated the expression of p-Akt and p-mTOR. The N2a cells were treated with PSP for 24 h. (c) The phosphorylation of Akt, mTOR, p70S6K, and p-4E-BP1 by the stimulation of PSP were blocked after the pretreatment of inhibitors LY294002 (10 μM) and rapamycin (250 nM) alone or in combination. Quantification of relative protein levels is shown in the right panel (). , , and by Student’s -test.

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