Berbamine Suppresses the Progression of Bladder Cancer by Modulating the ROS/NF-κB Axis

Figure 6

Berbamine exerted antitumor activity against bladder cancer cells by modulating the ROS/NF-κB axis. 5637 and T24 cells were treated with 32 μM berbamine in the presence or absence of 10 mM NAC. (a, b) Representative images of the ROS generation level were captured using a fluorescence microscope. (c, d) Flow cytometry was performed to measure cell apoptosis. (e, f) The levels of Bcl-2 and Bax proteins were measured by western blotting. (g, h) The levels of P65 and P-P65 proteins were measured by western blotting. Values are represented (all dates are expressed) as the . The experiment was repeated at least three times. Statistical significance was determined using two-tailed Student’s-test or one-way ANOVA. ; ; ; ^#; ^##.

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