Research Article
Emodin-Induced Oxidative Inhibition of Mitochondrial Function Assists BiP/IRE1α/CHOP Signaling-Mediated ER-Related Apoptosis
Figure 6
Oxidation-related decrease in mitochondrial function participated in ER stress-related apoptosis. (a) The mtROS levels in L02 cells treated with DOX were measured using the MitoSOX probe. (b–e) The expressions and quantification analysis of BiP, IRE1α, CHOP, and Caspase-3 in cells treated with TM and DOX. (f) The apoptosis rates of L02 cells treated with DOX, TM, or DOX+TM were analyzed using FACS analysis. (g) Mitochondrial functions in the L02 cells treated with TM, DOX, or TM + DOX for 24 h, and the effects of these treatments on OCRs of basal and ATP production. , , and vs. the control groups.
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