P2X7 Receptor Induces Pyroptotic Inflammation and Cartilage Degradation in Osteoarthritis via NF-κB/NLRP3 Crosstalk

Figure 7

Staining evaluations of inhibitor treatments and P2X7 activation in knee-joint cartilage of MIA-induced OA rats. Cartilage and subchondral bone of knee-joints were stained using (a) H&E and (b) Toluidine Blue O (scale bar: 500 μm). (c) Quantitative analysis of OARSI score from (a, b). (d) TUNEL staining of sagittal central sections of the cartilage in each group (scale bar: 50 μm). (f) Quantitative data for (d). (e) IHC staining of P2X7, MMP13, collagen II, NF-κB p65, NLRP3, IL-1β, and caspase-1 (scale bar: 50 μm). (g) Quantitative analysis of immune-positive cells. Data are presented as of at least three independent experiments. , .