Pyrogallol-Phloroglucinol-6 6-Bieckol on Attenuates High-Fat Diet-Induced Hypertension by Modulating Endothelial-to-Mesenchymal Transition in the Aorta of Mice

Figure 4

Comparative analysis of ECE and PPB administration on the reduction of EndMT in HFD-fed mice. (a) Confocal microscopic images demonstrating EC marker, PECAM-1 (arrow, top panel; green) and vWF (arrow, bottom panel; green), and nuclei (DAPI, blue) in the aorta of HFD-fed mice. Quantitative graphs demonstrating (b) the percentage of PECAM-1-positive cell (% of DAPI) and (c) vWF-positive cell (% of DAPI) from representative images. Scale  μm. (d) Confocal microscopic images demonstrating EndMT-related markers, α-SMA (arrow, top panel; green) and vimentin (arrow, bottom panel; green), and nuclei (DAPI, blue) in the aorta of HFD-fed mice. Scale  μm. Quantitative graphs demonstrating (e) the percentage of α-SMA-positive cell (% of DAPI) and (f) vimentin-positive cell (% of DAPI) from representative images. vs. NFD/saline, ^#vs. HFD/saline.

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