Quercetin Reverses Cardiac Systolic Dysfunction in Mice Fed with a High-Fat Diet: Role of Angiogenesis

Figure 5

High-fat diet increases oxidative stress but not senescence in the myocardium of C57BL/6J mice; quercetin attenuated myocardial oxidative stress. Data are . (a) Representative immunofluorescence images labels with 8-OHdG in the heart. Control+vehicle (CV; ); control+quercetin (CQ; ); high-fat+vehicle (HV; ); high-fat+quercetin (HQ; ). (b) Cardiac colorimetric (blue) staining of senescence-associated β-galactosidase (SA-β-Gal). CV, . CQ, . HV, . HQ, . (c) Cardiac fluorescent SPiDER-β-Gal staining. Neither was different among the groups. CV, . CQ, . HV, . HQ, . (d) Cardiac p16, p21, p53, and Active-A gene expression. Expression of p16 and p53 genes was upregulated only in HQ. CV, . CQ, . HV, . HQ, . vs. CV. ^# vs. CQ. ^{^} vs. HV.

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