Decitabine Downregulates TIGAR to Induce Apoptosis and Autophagy in Myeloid Leukemia Cells

Figure 2

DAC induces intracellular ROS production by downregulating TIGAR. HL-60 and K562 cells were treated with different doses of DAC for 24 hours. (a, b) The production of ROS was assessed by FCM. (c, d) NADPH levels were detected by the NADPH detection kit. The NADPH (e) and ROS (f) levels in K562 cells, with or without TIGAR overexpression, and HL-60 cells, with or without TIGAR knockdown, when compared with CD34+ cells. The values were presented as the (SD) of three independent experiments. , , NS compared to the control group.

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