Research Article
Hydrogen Sulfide Is a Regulator of Hemoglobin Oxygen-Carrying Capacity via Controlling 2,3-BPG Production in Erythrocytes
Figure 3
The effects of H2S on 2,3-BPG concentration and Hb-O2 affinity in cultured erythrocytes. (a–c) Isolated mouse erythrocytes were treated with the indicated doses of GYY4137 for 6 h and then harvested and used for determination of the 2,3-BPG level (a) and oxygen dissociation curve ((b) P50 value; (c) representative trace of the oxygen dissociation curve). Data are expressed as ( cultures). (d–f) After 6 h treatment of human erythrocytes with GYY4137, the cells were harvested for determination of the 2,3-BPG level (d) and oxygen dissociation curve ((e) P50 value; (f) representative trace of the oxygen dissociation curve). Data are expressed as ( cultures). , , and .
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