Research Article
Hydrogen Sulfide Is a Regulator of Hemoglobin Oxygen-Carrying Capacity via Controlling 2,3-BPG Production in Erythrocytes
Figure 5
The effects of H2S on BPGM trafficking between the membrane and the cytoplasm in cultured erythrocytes. Erythrocytes were isolated from WT mice (a, b, e) and healthy volunteers (c, d, e) and then incubated with the indicated doses of GYY4137 for 6 h. The cells were harvested for measurement of cytosolic BPGM activity, determination of membrane and cytosolic BPGM levels, and immunofluorescent staining of BPGM. (a, c) Cytosolic BPGM activity in mouse (a) and human (c) RBCs with and without GYY4137 treatment. (b, d) Membrane and cytosolic BPGM levels in mouse (b) and human (d) erythrocytes with and without GYY4137 treatment. (e) Representative images of staining in mouse and human erythrocytes with and without GYY4137 treatment. Data are expressed as ( cultures). , , , and .
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