Research Article
Hydrogen Sulfide Is a Regulator of Hemoglobin Oxygen-Carrying Capacity via Controlling 2,3-BPG Production in Erythrocytes
Figure 6
The effects of H2S on the interaction of Hb and BPGM with membrane proteins and Hb trafficking in erythrocytes. (a) Interaction of Hb and BPGM with band 4.2 protein. Coimmunoprecipitation with band 4.2 antibody was performed in mouse RBC lysates. BPGM and Hb in the immunoprecipitate were detected by western blot analysis. (b) The effects of H2S on Hb trafficking between the membrane and the cytoplasm in vivo. WT and Cse-/- mice were administered with saline or GYY4137 (50 mg/kg). Erythrocytes were obtained for the measurement of membrane heme concentrations 24 h after treatment. Data are expressed as (). (c, d) The effects of H2S on Hb trafficking between the membrane and the cytoplasm in cultured erythrocytes. Isolated mouse (c) or human (d) erythrocytes were treated with the indicated doses of GYY4137 for 6 h and then were harvested for the measurement of membrane heme concentrations. Data are expressed as ( cultures). (e) Representative images showing the effects of H2S on the interaction of Hb and BPGM with band 4.2 protein. WT and Cse-/- mice were injected with saline or GYY4137 (50 mg/kg). Blood samples were collected 24 h after treatment. Erythrocytes were isolated for the coimmunoprecipitation analysis. , , and .
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