Cicadidae Periostracum Attenuates Atopic Dermatitis Symptoms and Pathology via the Regulation of NLRP3 Inflammasome Activation

Figure 4

CP treatment effect on TSLP secretion and mRNA expression in a house dust mite-induced AD mouse model. (a) TSLP serum levels were measured by ELISA. (b) The relative expression of TSLP was analyzed by real-time PCR, in dorsal and ear skin samples, normalized to GAPDH. (c) TSLP levels were also evaluated locally, by immunofluorescence analysis, for evaluation in dorsal and ear skin samples.. Quantitative analysis for (d) dorsal and (e) ear skin TSLP levels is also represented. Dexamethasone (Dex) was used as a positive control. All data are represented per group, as of three independent experiments. Statistical differences were evaluated using the one-way ANOVA test, with Dunnett’s post hoc analysis, and are represented as follows: ^## and ^### compared with the negative control group (NC); , , and compared with the untreated control group (HDM).

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