Cicadidae Periostracum Attenuates Atopic Dermatitis Symptoms and Pathology via the Regulation of NLRP3 Inflammasome Activation

Figure 5

CP treatment affects NLRP3 inflammasome activation in a house dust mite-induced AD mouse model. (a) NLRP3 inflammasome components were analyzed in dorsal skin samples by Western blot. Bar graphs represent the relative expression of (c) NLRP3, (d) ASC, (e) cleaved caspase-1, and (f) IL-1β. (b) NLRP3 inflammasome components were also analyzed in ear skin samples. Bar graphs represent the relative expression of (g) NLRP3, (h) ASC, (i) cleaved caspase-1, and (j) IL-1β. Relative expression levels were obtained by normalization against β-actin. Dexamethasone (Dex) was used as a positive control. All data are represented per group, as of three independent experiments. Statistical differences were evaluated using the one-way ANOVA test, with Dunnett’s post hoc analysis, and are represented as follows: ^## and ^### compared with the negative control group (NC); , , and compared with the untreated control group (HDM).

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