Cicadidae Periostracum Attenuates Atopic Dermatitis Symptoms and Pathology via the Regulation of NLRP3 Inflammasome Activation

Figure 6

CP treatment effect on the expression of inflammatory cytokines in a house dust mite-induced AD mouse model. The relative expression of Th1/Th2 inflammatory cytokines was analyzed by real-time PCR in dorsal and ear skin samples. Values were normalized to GAPDH expression levels. Normalized results for (a) IL-1β, (b) IL-4, (c) IL-5, (d) IL-6, (e) IL-8, (f) IL-13, (g), TNF-α, and (h) IFN-γ expression in dorsal skin samples are shown. Normalized results for (i) IL-1β, (j) IL-4, (k) IL-5, (l) IL-6, (m) IL-8, (n) IL-13, (o) TNF-α, and (p) IFN-γ expression in ear skin samples are also represented. Dexamethasone (Dex) was used as a positive control. All data are represented per group, as of three independent experiments. Statistical differences were evaluated using the one-way ANOVA test, with Dunnett’s post hoc analysis, and are represented as follows: ^#, ^##, and ^### compared with the negative control group (NC); , , and compared with the untreated control group (HDM).