Melatonin Attenuates Diabetic Myocardial Microvascular Injury through Activating the AMPK/SIRT1 Signaling Pathway

Figure 4

Impacts of compound C and SIRT1 siRNA on the melatonin-stimulated AMPK/SIRT1 signaling in CMECs. (a) p-AMPK Thr¹⁷², AMPK, and SIRT1 expressions were assessed using western blot in CMECs treated with compound C. (b–d) Quantitative analysis of p-AMPK Thr¹⁷², AMPK, and SIRT1 expressions. (e) p-AMPK Thr¹⁷², AMPK, and SIRT1 expressions were determined using western blot in CMEC transfection with SIRT1 siRNA. (f–h) Quantitative analysis of p-AMPK Thr¹⁷², AMPK, and SIRT1 expressions. Data were expressed as the (). vs. NG, ^# vs. HG, ^† vs. HG+Mel, ^## vs. HG+siNC, and ^†† vs. HG+siNC+Mel. NG: normal glucose; HG: high glucose; Mel: melatonin; Com. C: compound C; NC: negative control.

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