Melatonin Attenuates Diabetic Myocardial Microvascular Injury through Activating the AMPK/SIRT1 Signaling Pathway

<div>Inhibition of AMPK with compound C abrogated the beneficial effects of melatonin against oxidant stress and apoptosis in HG-cultured CMECs. (a) Flow cytometry analysis of ROS level by DCFH-DA probe. (b) ROS mean fluorescent intensity. (c) MDA concentrations. (d) SOD activity. (e) Apoptosis of Annexin V-FITC/PI to evaluate apoptosis. (f) Quantification histograms indicated the apoptotic percentage. (g) Western blot to estimate Bcl-2 expression. (h) Representative pictures of JC-1 staining (magnification, 400x). 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NG: normal glucose; HG: high glucose; Mel: melatonin; Com. C: compound C.</div>

Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: Melatonin Attenuates Diabetic Myocardial Microvascular Injury through Activating the AMPK/SIRT1 Signaling Pathway 