Melatonin Attenuates Diabetic Myocardial Microvascular Injury through Activating the AMPK/SIRT1 Signaling Pathway

Figure 6

Efforts of melatonin on SIRT1-silenced CMEC oxidant stress and apoptosis induced by HG in CMECs. (a) DCFH-DA to measure ROS. (b) ROS amounts quantified by mean fluorescence intensities. (c) Levels of MDA. (d) Activity of SOD. (e) Annexin V-FITC/PI to evaluate apoptosis extent. (f) Quantification histograms to indicate the apoptotic percentage. (g) Western blot to examine Bcl-2 expression. (h) Representative pictures of JC-1 staining (magnification, 400x). Data were expressed as the (). vs. NG, ^# vs. HG+siNC, and ^† vs. HG+siNC+Mel. NG: normal glucose; HG: high glucose; Mel: melatonin; NC: negative control.

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