Albumin Reduces Oxidative Stress and Neuronal Apoptosis via the ERK/Nrf2/HO-1 Pathway after Intracerebral Hemorrhage in Rats

Figure 3

Effects of albumin on short-term neurobehavioral outcome and neuronal damage at 72 h after ICH. (a) Albumin improved short-term neurological function (modified Garcia test, left forelimb placement test, and corner turn test) at 24 h and 72 h after ICH (/group). Short-term neurological results showed that albumin treatment at the medium-dosage of 1.25 g/kg improved short-term neurological function significantly compared to the vehicle and low and high dosage groups. Therefore, we further chose the middle dosage for further study. (b) Representative micrographs of FJC-positive cells within the ipsilateral perihematomal region at 72 h after ICH. (c) Representative micrographs of TUNEL-positive neurons within the ipsilateral perihematomal region at 72 h after ICH. (d) Brain sample with schematic illustration showing one areas in the perihematomal region and the small white square in the coronal section of the brain indicates the area used for counting FJC and TUNEL-positive neurons. (e, f) Quantitative analyses of TUNEL and FJC-positive cells in the perihematomal area at 72 h after ICH (/group); scale bar = 50 μm, nuclei were stained with DAPI (blue). vs. sham, ^@ vs. ICH+vehicle, , one-way ANOVA, Tukey’s test, TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling; FJC: Fluoro-Jade C staining.

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