Albumin Reduces Oxidative Stress and Neuronal Apoptosis via the ERK/Nrf2/HO-1 Pathway after Intracerebral Hemorrhage in Rats

Figure 4

Effects of albumin on oxidative stress levels at 72 h after ICH. (a) Representative micrographs of 8-OHdG (green) immunofluorescence staining in the ipsilateral perihematomal region at 72 h after ICH. Nuclei were stained with DAPI (blue). (b) Brain sample with schematic illustration showing one areas in the perihematomal region and the small white square in the coronal section of the brain indicates the area used for counting 8-OHdG-positive cells. (c) Quantitative analysis of 8-OHdG (green) immunofluorescence staining in the ipsilateral perihematomal region at 72 h after ICH (/group). (d) Superoxide dismutase (SOD) and malondialdehyde (MDA) levels (/group). vs. sham, ^@ vs. ICH+vehicle, , one-way ANOVA, Tukey^’s test. 8-OHdG: 8-hydroxy-2-deoxyguanosine; SOD: superoxide dismutase; MDA: malondialdehyde.

(a)

(b)

(c)

(d)