Albumin Reduces Oxidative Stress and Neuronal Apoptosis via the ERK/Nrf2/HO-1 Pathway after Intracerebral Hemorrhage in Rats

Figure 6

Blockade of ERK and Nrf2 reversed the effects of albumin on short-term neurobehavioral outcome and oxidative stress-induced neuronal death at 72 h after ICH. (a) Modified Garcia test, left forelimb placement test, and corner turn test. (b) Representative western blotting images. (c–h) Quantitative analyses of p-ERK/ERK, Nrf2, HO-1, Romo1, Bax, and Bcl-2 expression. vs. sham, ^@ vs. ICH+vehicle, ^& vs. ICH+albumin, ^# vs. ICH+albumin+DMSO; , one-way ANOVA, Tukey’s test, /group. p-ERK1/2: phosphorylated extracellular regulated protein kinases 1/2; ERK1/2: extracellular regulated protein kinases1/2; Nrf2: nuclear factor-E2-related factor 2; HO-1: heme oxygenase 1; Romo1: reactive oxygen species modulator 1; Bax: B-cell lymphoma-2 associated X protein; Bcl-2: B-cell lymphoma-extra large 2.

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