Trehalose Augments Neuron Survival and Improves Recovery from Spinal Cord Injury via mTOR-Independent Activation of Autophagy

Figure 3

Effect of trehalose on mitochondrial damage-induced apoptosis in neurons after SCI. (a) Immunostaining of cleaved CASP3 in neurons in the lesion of the three different groups (control, SCI, and SCI+TRE) on day 3; scale bar: 40 μm; (b) Quantitative analysis of cleaved CASP3 density in neurons of each group. (c, e, g, and i) Western blot analysis of the protein levels of cleaved PARP, Bax, cleaved CASP3, and CYCS in each group on day 3. (d, f, h, and j) Quantitative analysis of cleaved PARP, Bax, cleaved CASP3, and CYCS in the three groups. in each group. and for comparisons with the control rats, ^# and ^## for comparisons between SCI rats.

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