Research Article
Lactobacillus plantarum Exhibits Antioxidant and Cytoprotective Activities in Porcine Intestinal Epithelial Cells Exposed to Hydrogen Peroxide
Figure 3
Lactobacillus plantarum ZLP001 improves the H2O2-induced redox state in IPEC-J2 cells. IPEC-J2 cells were incubated with or without 106 CFU L. plantarum ZLP001 for 3 h; after which, the medium was replaced with a fresh medium containing 1,000 μM H2O2, and the cells were further incubated for 4 h. (a) L. plantarum ZLP001 prevents H2O2-induced ROS accumulation in IPEC-J2 cells as indicated by DCFH-DA staining. (b) Quantification of the ROS levels based on DCFH-DA fluorescence. (c) Effect of L. plantarum ZLP001 on the mitochondrial membrane potential (MMP) in H2O2-induced IPEC-J2 cells as analyzed by flow cytometry. (d) Quantification of the effect on the MMP based on flow-cytometric data. (e) Concentrations of glutathione (GSH) and oxidized glutathione (GSSG) were determined using GSSG and GSH detection assays. (f) Concentrations of malondialdehyde (MDA) were determined using an MDA assay kit. All data represent the of three independent experiments. Means were compared one-way ANOVA followed by Duncan’s post hoc tests. .
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