Effect and Mechanism of LRP6 on Cardiac Myocyte Ferroptosis in Myocardial Infarction

Figure 3

LRP6 deletion regulates ferroptosis in cardiomyocytes through autophagy. (a) The presented blots were obtained from the expression of autophagy-related proteins LC3-A/B, ATG, and p62 after LRP6 interference in the cardiomyocytes treated with hypoxia and erastin. Top: typical blots. Pooled data: biological replication indicated five mice in each group. (b) Effect of ATG5 siRNA mediated the expression of ATG5 in cardiomyocytes. Pooled data: biological replication indicated five mice in each group. Data were and compared with ctrl by one-way ANOVA with Bonferroni’s post hoc test. (c) Analysis of the cardiomyocytes with LRP6 siRNA and ATG5 siRNA influence on survival rate in cardiomyocytes treated with hypoxia and erastin. Statistical analysis was obtained from five mice in each group. (d, e) In this case, the presented Fe²⁺ and MDA levels were induced by LRP6 deletion. Pooled data: biological replication indicated five mice in each group. Data were and compared with ctrl or si-LRP6 by one-way ANOVA with Bonferroni’s post hoc test.

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