The Roles of FGF21 and ALCAT1 in Aerobic Exercise-Induced Cardioprotection of Postmyocardial Infarction Mice

Figure 4

Aerobic exercise and knockout alcat1 reduced cardiac oxidative stress and apoptosis in MI mice. (a) Myocardial infarct size was measured with Masson’s trichrome staining. The images showed that the fibrosis marker was stained in blue, whereas cardiac muscle fibers in red and nuclei in dark brown. Scale bar: 200 μm. Quantification of collagen volume of fraction (CVF%). (b) Echocardiography. (c) Left ventricular end diastolic diameter (LVIDd). (d) Left ventricular systolic diameter systole (LVIDs). (e) Left ventricular ejection fraction (EF). (f) Left ventricular short axis (FS). (g) Protein expression levels of ALCAT1. (h) MDA content in the myocardium. (i) GSH content in the myocardium. (j) GSH-Px activity in the myocardium. (k) CAT activity in the myocardium. (l) T-SOD activity in the myocardium. (m) TUNEL staining: TUNEL-positive granules in red and DAPI in blue. (n) TUNEL-positive cells count. (o) Protein expression levels of the Bax/Bcl-2 ratio. CVF: collagen volume fraction; EF: ejection fraction%; FS: fractional shortening%; LVIDd; left ventricular internal diameter diastole; LVIDs: left ventricular internal diameter systole. S: sham group; ME: MI + aerobic exercise group. Data presented are . One-way ANOVA with post hoc LSD multiple-comparison test. , .

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