The Roles of FGF21 and ALCAT1 in Aerobic Exercise-Induced Cardioprotection of Postmyocardial Infarction Mice

Figure 5

AICAR or rhFGF21 reduced the oxidative stress and ER stress-induced apoptosis by H₂O₂ in H9C2 cells. (a) T-SOD activity in H9C2 cells. (b) MDA in H9C2 cells. (c–f) Western blotting images and their densitometric quantitative analysis of ALCAT1, p-PI3K/t-PI3K ratio, p-AKT/t-AKT ratio, p-IRE1α/t-IRE1α ratio, p-JNK/t-JNK ratio, GRP78, CHOP, Bax/Bcl-2 ratio, and c-caspase3. (g) TUNEL staining: TUNEL-positive granules in red and DAPI in blue. (h) TUNEL-positive cells count. Data presented are . One-way ANOVA with post hoc LSD multiple-comparison test. , .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)