Geniposide Attenuates Hyperglycemia-Induced Oxidative Stress and Inflammation by Activating the Nrf2 Signaling Pathway in Experimental Diabetic Retinopathy

Figure 2

The effects of geniposide (GEN) on viability, Nrf2 nuclear translocation, and ROS accumulation in HG-stimulated Müller cells. Müller cells were exposed to different concentrations of GEN with or without HG. (a, b) The viability of Müller cells was measured by CCK-8 assays. vs. the control group. (c, d). The protein expression of Nrf2 in the nucleus and cytoplasm was measured by western blotting. H3 and β-actin were used as nuclear and cytoplasmic loading controls, respectively. and vs. the NG group and ^# vs. the HG group. (e, f) The protein expression of HO-1 and NQO1 was measured by western blotting. vs. the NG group and ^# vs. the HG group. (g) ROS production was measured by an ROS assay kit. /group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)