[Retracted] MicroRNA-133b Inhibition Restores EGFR Expression and Accelerates Diabetes-Impaired Wound Healing

Figure 5

miR-133b is a potential regulator of EGFR expression. (a) Identification of miR-133b as a putative regulator of EGFR expression based on miRTarBase, TargetScan, and miRWalk target prediction analyses. (b, c) Luciferase reporter assay demonstrating that miR-133b binds to the 3UTR of the EGFR mRNA. (d) Relative miR-133b levels in control and agomiR-NC-, agomiR-133b-, antagomiR-NC-, and antagomiR-133b-transfected HUVECs (qRT-PCR). (e) qRT-PCR for assessing the effect of miR-133b on EGFR expression. (f) Changes in relative miR-133b levels following exposure of HUVECs to 20 mM D-glucose for 4 and 24 h. (g, h) Relative miR-133b expression in wounds from nondiabetic (g) and diabetic mice (h) at 0, 3, and 7 d postwound induction.

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