MKRN1 Ubiquitylates p21 to Protect against Intermittent Hypoxia-Induced Myocardial Apoptosis

Figure 2

IH promotes myocardial apoptosis and downregulates MKRN1. (a) HE staining of heart tissue sections (scale bar, 100 μm); the blue arrow shows myocardial structure being disorganized; the black arrow shows infiltration of inflammatory cells; (b) representative image of myocardial apoptosis detected using TUNEL staining (scale bar, 20 μm) and quantification of TUNEL-positive cell nuclei (); (c) representative immunoblotting analysis and quantification of MKRN1, Bcl-2, Bax, Bak, p21, cleaved caspase-3, caspase-3 protein levels, and Bax/Bcl-2 ratio in the heart () and GAPDH as an internal control; (d) IHC staining for Bcl-2, Bax, Bak, caspase-3, cleaved caspase-3, and p21 proteins in the heart (scale bar, 100 μm); (e) MKRN1 mRNA expression in the heart was detected using qPCR (); (f) IHC staining for MKRN1 expression in the heart (scale bar, 100 μm). indicates the independent sample number in each group. .

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