MKRN1 Ubiquitylates p21 to Protect against Intermittent Hypoxia-Induced Myocardial Apoptosis

Figure 3

IH induces myocardial apoptosis and downregulates MKRN1 H9C2 and AC16 cells were subjected to NC or IH stimulation. (a) Representative immunoblotting analysis and quantification of Bcl-2, Bax, Bak, p21, cleaved caspase-3, caspase-3 protein levels, and Bax/Bcl-2 ratio () and GAPDH as an internal control; (b) representative immunoblotting analysis and quantification of MKRN1 protein expression () and GAPDH as an internal control; (c) MKRN1 mRNA expression was detected using qPCR (). indicates the independent sample number in each group. .

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