MKRN1 Ubiquitylates p21 to Protect against Intermittent Hypoxia-Induced Myocardial Apoptosis

Figure 4

MKRN1 overexpression reduced IH-induced ROS production and myocardial apoptosis. H9C2 and AC16 cells transfected with MKRN1-overexpressing lentiviruses or empty virus were subjected to NC or IH stimulation. (a) MKRN1 mRNA expression was detected using qPCR (); (b, c) representative immunoblotting analysis (b) and quantification of MKRN1, Bcl-2, Bax, Bak, p21, cleaved caspase-3, caspase-3 protein levels, and the Bax/Bcl-2 ratio (c) () and GAPDH as an internal control, and versus the group; versus the group; (d) representative fluorescence image of DHE staining and quantification of fluorescence intensity (scale bar, 100 μm) (), and versus the group; versus the group; versus the group. indicates the independent sample number in each group.

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