Neuroprotective Effect of Ceftriaxone on MPTP-Induced Parkinson’s Disease Mouse Model by Regulating Inflammation and Intestinal Microbiota

Figure 6

Ceftriaxone regulated intestinal tight junction proteins and inhibited intestinal inflammation and systemic inflammation caused by MPTP. (a) Western blot analysis of permeation-related proteins ZO-1, Occludin expression in the colon of mice, β-actin was used as an internal control. (b) Western blotting of TLR-4, MyD88, p-NFκB, and NFκB expression in in the colon of mice, β-actin was used as an internal control. (c) The expression of inflammatory cytokines TNF-α IL-6 and IL-1β in protein levels was detected by ELISA. C group (), control group; M group (), MPTP group; MCEF group (), MPTP + ceftriaxone group; MCEFF group (), MPTP + ceftriaxone + fecal microbiota transplantation group; FMT group (), fecal microbiota transplantation group, FCEF group (), fecal microbiota transplantation group + ceftriaxone group. Data are presented as means ± SD. One-way repeated-measures ANOVA with Tukey’s test for multiple comparisons (a–c, respectively); , . CEF: ceftriaxone; MPTP: 1-methyl-4-phenyl-1, 2, 3, 6-tetra-hydropyridine.

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